DNA Vectors
IBA LifeSciences StrGate Acptr Vctr pASG-IBA44
The pASG-IBA44 vector is designed for expression of recombinant proteins with an N-terminal Strep-tagII and a C-terminal His-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA3
The pASG-IBA3 vector is designed for expression of recombinant proteins with a C-terminal Strep-tagII in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
Cayman Chemical EGFP mRNACap-1mo5U 100ug
An mRNA for the expression of EGFP; encapsulation in LNPs can be used for mRNA delivery and expression of EGFP in vitro or in vivo
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt1
The pASG-IBAwt1 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
New England Biolabs, Inc. PhiX174 Virion DNA – 250 µg
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10^6 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.
System Biosciences LLC PGREENFIRE1-LXRE IN LXR ALPHA
pGreenFire1-LXRE in LXR alpha (HepG2 cell line)
New England Biolabs, Inc. M13K07 Helper Phage – 1.8 ml
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
System Biosciences LLC Cre Recombinase Expression Plasmid (pCMV-CRE) | 10ug
With the Cre-LoxP site-specific recombination system used in most of our HR Targeting Vectors, you can get rid of extra sequences, such as selection cassettes, by transiently expressing the Cre recombinase from the pCMV-CRE Cre Recombinase Expression Plasmid.